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PrimePlex Multiplex ImmunoAssay - March 2019 Publication
24 Apr 2019

PRIMEPLEX Multiplex MAP Kit Validation and Quality Assurance Criteria

We carefully develop and validate every multiplex kit and evaluate performance criteria including cross-reactivity, sensitivity, specificity, dilution linearity, kit stability and sample behavior (e.g. detectability and stability). We write the protocol with assay performance and your workflow in mind, making it easy to find the information you need.

Each multiplex kit includes all necessary reagents to prepare the assay to read on your Luminex® instrument, just add your sample and water:

  • Selectable capture antibody-conjugated magnetic beads in solution
  • A premixed standard cocktail allowing you to easily rehydrate and prepare your serial dilutions
  • Two premixed quality control cocktails (QCs) to qualify assay performance
  • A premixed detection antibody cocktail designed to yield consistent analyte profiles within panel
  • A prediluted optimized concentration of Streptavidin Phycoerythrin (SAPE)
  • An optimized serum matrix is included for kits validated for serum or plasma samples, to mimic native sample environment
  • Bead diluent containing proprietary blocking reagents to reduce non-specific binding to the antibodies in the assay
  • 10X wash buffer and assay buffer optimized specifically for the kit
  • 96- or 384-well plate (depending on assay format) with two plate sealers

Multiplex kit cell signaling kits have a different format to support the use of cell lysate samples.