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ELISA Workshop at UM
26 May 2016
8:00am - 5:00pm
Medical Biotechnology Laboratory, Faculty of Medicine, UM
Organized by: 
Cell & Molecular Biology Laboratory (CMBL) & Prima Nexus Sdn Bhd

Types of ELISA Methods:

  •     Direct ELISA:

    Antigens are immobilized and enzyme-conjugated primary antibodies are used to detect or

    quantify antigen concentration. The specificity of the primary antibody is very important.

  •   PROS: minimum procedure; avoids cross-reactivity from secondary antibody.
  •   CONS: requires labeling of all primary antibodies - high cost; not every antibody is suitable for labeling.



  • Indirect ELISA:

    Primary antibodies are not labeled, but detected instead with enzyme-conjugated secondary

    antibodies that recognize the primary antibodies.

  •  PROS: secondary antibodies are capable of signal amplification; many available secondary antibodies can be used for different assays; unlabeled primary antibodies retain maximum immunoreactivity.
  •  CONS: cross-reactivity may occur.



  •     Sandwich ELISA:

    The antigen to be measured is bound between a layer of capture antibodies and a layer of

   detection antibodies. The two antibodies must be very critically chosen to prevent cross-

   reactivity or competition of binding sites.

  •   PROS: sensitive, high specificity, antigen does not need to be purified prior to use.
  •   CONS: antigens must contain at least two antibody binding sites.



  • Competitive ELISA:

    The antigen of interest from the sample and purified immobilized antigen compete for binding to

    the capture antibody. A decrease in signal when compared to assay wells with purified antigen

    alone indicates the presence of antigens in the sample.

  •   PROS: crude or impure samples may be used, high reproducibility.
  •   CONS: lower overall sensitivity and specificity.

  • Group Registration
  • Individuals Registration
Group Registration

Kindly fill in the details of your group members below, contact us if your group is more than 10 pax.

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