Error message

The spam filter installed on this site is currently unavailable. Per site policy, we are unable to accept new submissions until that problem is resolved. Please try resubmitting the form in a couple of minutes.

You are here

ELISA Workshop at UM
Date: 
26 May 2016
Time: 
8:00am - 5:00pm
Location: 
Medical Biotechnology Laboratory, Faculty of Medicine, UM
Organized by: 
Cell & Molecular Biology Laboratory (CMBL) & Prima Nexus Sdn Bhd

Types of ELISA Methods:

  •     Direct ELISA:

    Antigens are immobilized and enzyme-conjugated primary antibodies are used to detect or

    quantify antigen concentration. The specificity of the primary antibody is very important.

  •   PROS: minimum procedure; avoids cross-reactivity from secondary antibody.
  •   CONS: requires labeling of all primary antibodies - high cost; not every antibody is suitable for labeling.

 

 

  • Indirect ELISA:

    Primary antibodies are not labeled, but detected instead with enzyme-conjugated secondary

    antibodies that recognize the primary antibodies.

  •  PROS: secondary antibodies are capable of signal amplification; many available secondary antibodies can be used for different assays; unlabeled primary antibodies retain maximum immunoreactivity.
  •  CONS: cross-reactivity may occur.

 

 

  •     Sandwich ELISA:

    The antigen to be measured is bound between a layer of capture antibodies and a layer of

   detection antibodies. The two antibodies must be very critically chosen to prevent cross-

   reactivity or competition of binding sites.

  •   PROS: sensitive, high specificity, antigen does not need to be purified prior to use.
  •   CONS: antigens must contain at least two antibody binding sites.

 

 

  • Competitive ELISA:

    The antigen of interest from the sample and purified immobilized antigen compete for binding to

    the capture antibody. A decrease in signal when compared to assay wells with purified antigen

    alone indicates the presence of antigens in the sample.

  •   PROS: crude or impure samples may be used, high reproducibility.
  •   CONS: lower overall sensitivity and specificity.



    CLICK HERE FOR IMAGE LIBRARY
  • Group Registration
  • Individuals Registration
Mr./Ms./Dr./AP./Prof./Datuk/Dato/TanSri
Group Registration

Kindly fill in the details of your group members below, contact us if your group is more than 10 pax.

Add 2nd person
Mr./Ms./Dr./AP./Prof./Datuk/Dato/TanSri