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REAL TIME PCR WORKSHOP -Real Time PCR for Gene Expression Analysis-
Date: 
07 Dec 2017 to 08 Dec 2017
Time: 
5:30pm
Location: 
UKM Medical and Molecular Biology Institute (UMBI)
Organized by: 
UKM Medical and Molecular Biology Institute (UMBI)

 

The workshop is designed for users who are new to real time quantitative PCR (qPCR), but all users are welcome. User will experience extracting RNA from blood and cells, performing RNA QC, converting RNA to cDNA, optimizing a qPCR reaction, assessing primers efficiency and determining expression level of their genes of interest. Clients will gain experience using DNA binding dyes chemistries. Lectures will include an overview of RNA extraction, gene expression, assay design and optimization, template preparation, analysis of results, additional applications of real-time PCR technology, and many more. Data analysis and simple statistical test will be included and will produce publication-ready figures. Some previous experience using standard PCR or real time PCR is helpful but not necessary for workshop attendees. 

 

OBJECTIVES
  • To give participants a combination of lecture and hands-on laboratory experience using qPCR methods
  • To expose and introduce a basic concept of qPCR and the analysis involved
  • To provide the fundamentals of cDNA synthesis, quality controls in qPCR
  • To promote awareness of application of qPCR in research
 
WORKSHOP FEE
 
RM800.00 (non-profit/ universities/government institution)
RM1000.00 (industry/profit-based organization)
 
*Fees inclusive of training materials, certificates and meals
 
AGENDA

 

  • cDNA Synthesis
  • Relative quantification qPCR (Gene Expression)
  • Optimizing a qPCR reaction
  • Data and statistical analysis

 

FULL WEBSITE: http://www.ukm.my/umbi/qpcr2017/