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2nd Fundamental Cell Culture and MTT Assay Demonstration Workshop
Date: 
12 Mar 2019 to 13 Mar 2019
Time: 
9:00am - 4:30pm
Location: 
Human Anatomy Lab, UPM
Organized by: 
Prima Nexus

The workshop provides a solid basis for the understanding of the growth of animal cells and combines lecture and laboratory exercises aimed at giving participants a deeper understanding of serum-free media applications and related topics. The two-day course will constitute theoretical classes and demonstration training. The contents of the workshop are as follows:

  • Principle, concept and application of cell culturing techniques

  • Lab maintenance and subculturing of cells

  • Preservation and cell revival

  • Cytotoxicity assay (MTT assay)

  • Biosafety and clinical waste management

WORKSHOP HIGHLIGHT

  • A platform to understand the handling of cells in vitro

  • Learning to perform cell viability assays

  • Hands on training on performing cytotoxicity

  • Training on biosafety and clinical waste management

TOPIC THAT WILL COVER

  • Oveview of Cell Culture Technique

  • Aseptic Techniqu & Good Cell Culture Practice

  • Cell Culture Lab Management

  • Resuscitation of Frozen Cell Lines

  • Resuscitation of Frozen Cell Lines

  • Subculture of Adherent Cell Lines

  • Safety Aspects of Cell Culture

  • Managing Cell Culture

  • Cytotoxicity Assay (MTT assay)

PRACTICAL TOPIC

  • Cell Thawing and cell freezing

  • Cell line maintenance and subculture

  • Cell Counting by haemocytometer & cell scepter

  • Determination of mycoplasma contamination

  • MTT assay

WHO SHOULD ATTEND?

Science officer, research officer, young scientists, postdoctoral fellows, postgraduate students and individuals that have related work with cell culture, MTT Assay